Active air sampling requires the use of a microbiology air sampler to physically draw a known volume of air over or through a particle collection device and there are two main types.
Active air sampling sop.
Place the axis in vertical position.
5 2 operation of air.
Sterilise the stainless steel aspirating head of the air sampler prior to use by autoclaving as per sop.
6 2 2 after pressing the on off button the previously selected air sample volume retained in the memory appears on the display.
5 9 5 regulate the pressure of the air so that the flow rate is in between 140l to 180l per minute.
At 3 cm of the end of the axis screw a nut.
Analyze the microbial quality of air by sampling 100 litres of air at each of the locations mentioned in annexure i using active air sampler carry the pre incubated scda plates and sterile ss head in a ss container to the sampling location.
5 9 6 ensure that m air t media cassette is in place before sampling is started.
The following steps describe the way to assemble the passive air sampler.
The organisms are then visible to the naked eyes and can be counted for an assessment of the level of contamination.
This nut will be the bottom limit.
Fertility test of media.
5 9 7 close the media cassettes properly after sampling 5 9 8 clean the pump with 70 ipa before taking it to the next sampling location port.
1 impingers impingers use a liquid medium for particle collection.
6 1 6 place the cassette in the air sampler under an laf.
Perform the growth promotion test of each lot of media used for environmental monitoring as per sop.
Active and passive monitoring.
Active there are two primary methods for microbial air sampling.
Carry the preincubated scda plates and sterile ss head in a ss container to the sampling location.
Passive air sampling passive air sampling i e settle plates is a frequently.
In active monitoring a microbial air sampler is used to force air into or onto its collection medium e g petri dish with nutrient agar based test media over a specified period of time.
6 2 switching the sampler on.
Airborne gases and vapours are collected by a physical process such as diffusion through a static air layer or permeation through a membrane.
Enter into the aseptic area following aseptic area entry and exit sop of the concerned block.
The collected culture can then be incubated and.
Active air sampling sites should be done at the same locations or as close as practical to avoid compro mising the other measure or the product integrity.
Active sampling involves the use of an air sampling pump to actively pull air through a collection device such as a filter.
Analyse the microbial quality of air by sampling 100 litre of air at each of the locations using active air sampler.
Typically sampled air is drawn by a suction pump through a narrow inlet tube into a small.
Standard operating procedures for the passive air sampling 2 2 pas assembling the pas should be assembled from the bottom to the top.
Passive sampling however does not require active air movement from a pump.
